NHS group empowers acridine ester NSP-DMAE-NHS 194357-64-7: an excellent connector for biomarkers

Efficient and stable labeling of biomolecules is a crucial step in achieving high-sensitivity detection in chemiluminescence immunoassay, molecular diagnostics, and biomedical research. Among numerous labeling techniques, NHS ester modified acridine esters, especially acridine ester NSP-DMAE-NHS, have become "excellent connectors" in the fields of protein, antibody, and enzyme labeling due to their excellent labeling efficiency and reaction specificity. Its core advantage lies in the NHS active groups it carries.

1, Analysis of NHS ester structure and reaction mechanism

NHS ester, also known as N-hydroxysuccinimide ester, is an active functional group formed by dehydration condensation of N-hydroxysuccinimide and carboxyl compounds. In the NSP-DMAE-NHS of acridine ester, this group is connected to the luminescent core through a stable ester bond, which does not interfere with the luminescent process and provides an ideal reaction endpoint for biological coupling.

Acridine ester NSP-DMAE-NHS powder

Its labeling mechanism is clear and efficient: in a weakly alkaline buffer environment, NHS esters can undergo nucleophilic substitution reactions with primary amines (- NH ₂) in protein molecules, forming stable amide bonds (- CONH -) and releasing N-hydroxysuccinimide. This process realizes the covalent connection between acridine ester and target protein, laying the foundation for subsequent chemiluminescence detection.

2, Why are NHS esters the preferred labeling strategy?

Although there are more than one functional group that can react with primary amines, NHS esters exhibit significant advantages in practical applications:

1. Rich reaction targets

Phenylamine is widely present at the N-terminus and lysine (Lys) side chains of proteins, and due to its positive charge at physiological pH, it is mostly distributed on the surface of proteins, with high accessibility and easy contact reaction with labeling reagents.

2. High nucleophilic reactivity and selectivity

Among the common functional groups in biological samples, primary amines have strong nucleophilicity, fast reaction rate and high efficiency with NHS esters, few side reactions, high purity of labeled products, and are conducive to maintaining biological activity.

3. Strong applicability and universality

Whether it is ELISA, chemiluminescence immunoassay (CLIA), or flow cytometry, acridine ester NSP-DMAE-NHS can meet the sensitivity requirements of different detection platforms by adjusting the labeling ratio and reaction time. The stability of the labeled product exceeds 6 months and supports high-throughput automated operations.

3, The key to efficient labeling: precautions for use

Although NHS ester reagents have high labeling efficiency, strict control of reaction conditions is still necessary to fully utilize their performance:

PH environment is crucial: It is recommended that the reaction of acridine ester NSP-DMAE-NHS be carried out in weakly alkaline buffer systems (such as phosphates, borates, HEPES) with pH 7.2-9.0. If the pH is too low, the reaction will be slow; If it is too high, it will exacerbate the hydrolysis of NHS esters and reduce labeling efficiency.

Avoid amine containing buffers: When using acridine ester NSP-DMAE-NHS for related experiments, it is important to avoid using buffers containing primary amines such as Tris and glycine, as they compete to consume NHS active groups. After the reaction is complete, this type of buffer can be used to quench the remaining active ester.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd., as an advantageous manufacturer of luminescent reagents, has a history of nearly 20 years since its establishment in 2005. With rich research and development experience, it currently sells luminescent reagents with different functional groups such as acridine ester NSP-DMAE-NHS, acridine ester NSP-SA, and acridine ester NSP-SA-NHS. Desheng has professional laboratories and technicians who can provide customized services according to customer requirements. It is your trusted supplier of chemiluminescence reagents. If you have any related procurement needs in the near future, please feel free to contact me!