Luminol 521-31-3 luminescent reagent: a comprehensive analysis from production to application

In the fields of chemical analysis, biological detection, and forensic science, chemiluminescence technology has become an indispensable research tool due to its high sensitivity, rapid response, and easy operation. As the earliest discovered chemiluminescent reagent, Luminol reagent(luminescent ammonia) used to rely on imports for a long time. However, in recent years, domestic manufacturers represented by Desheng have achieved breakthroughs in the entire chain from raw material synthesis to end products through technological innovation, promoting the localization process of domestic scientific research and industrial applications.

Luminol synthesis route

Luminol, chemical name 3-aminophthalazide, CAS number: 521-31-3, is synthesized from 3-aminophthalic acid as the starting material. The first step is dehydration of the raw material to generate 3-nitrophthalic anhydride, which has a wide range of applications in organic synthesis and alcohol determination. Then proceed with nitration reaction, which will simultaneously generate two products, 3-nitro-phthalic acid and 4-nitro-phthalic acid. Among them, 3-nitro-phthalic acid reacts with hydrazine to ultimately produce the target product luminol; 4-Nitro-phthalic acid can be used to prepare isoluminol. The entire synthesis route is logically clear, and each step lays the foundation for obtaining high-purity luminol.

luminol powder

Separation of intermediate by-products of luminol

During the synthesis of luminol, by-products are generated in the intermediate, which react with hydrazine to produce isoluminol. Therefore, it must be removed to ensure the purity of luminol. The key is to utilize the difference in water solubility between 3-nitro-phthalic acid and 4-nitro-phthalic acid. In 3-nitro-phthalic acid molecules, the nitro group has a significant effect on the adjacent carboxyl group, forming intramolecular hydrogen bonds with the carboxylic acid. In addition, the intramolecular hydrogen bonds between adjacent dicarboxylate groups greatly inhibit the dissociation of carboxylic acid molecules, leading to a decrease in their water solubility. On the contrary, in 4-nitro-phthalic acid, the nitro group is difficult to form intramolecular hydrogen bonds with carboxylic acid, and has a high degree of dissociation in water and good water solubility. Based on this characteristic, appropriate separation methods can effectively remove by-products and obtain high-purity luminol.

The use of luminol luminescent reagent

Luminol belongs to enzymatic chemiluminescence reagents, and its luminescence reaction requires specific conditions. When detecting, it is necessary to utilize the catalytic effect of peroxidase HRP and add hydrogen peroxide in an alkaline environment to stimulate the chemiluminescence reaction. The uniqueness of HRP lies in its ability to label proteins or antibodies, which enables luminol to tightly bind to immune responses. This feature has brought great convenience to fields such as biomedical detection, for example, in immune analysis, the presence and content of specific antigens or antibodies can be accurately detected.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd., as a company deeply engaged in the research of chemiluminescence reagents for many years, relies on rich experience and advanced technology to not only provide high-quality luminol, but also produce various luminescent reagents such as isoluminol and acridine esters. These reagents have reached the international advanced level in terms of performance, providing strong support for domestic scientific research and testing work. If you need related products in the near future, you can click on the official website of Desheng to learn more details or contact me!