Recombinant glycerol kinase: Breaking through clinical testing bottlenecks and leading the upgrade of triglyceride assay kits

In clinical biochemical testing, triglyceride (TG) content is an important basis for the diagnosis of cardiovascular diseases, and glycerol kinase (GK), as the core biological enzyme for detection, its characteristics and applications directly affect the accuracy of results. This article analyzes the basic characteristics, clinical value, requirements for enzyme-linked immunosorbent assay kits, and advantages of recombinant enzymes of glycerol kinase.

1, Basic molecular characteristics of glycerol kinase

Glycerol kinase is a structurally clear protein with a stable molecular weight of 55KDa, composed of 502 amino acid residues, and its biological activity is determined by specific molecular composition. According to enzymatic classification, it is numbered E.C 2.7.1.30 and belongs to the phosphotransferase class of transferases. Its core function is to catalyze the phosphorylation reaction of glycerol and ATP, generating glycerol-3-phosphate and ADP. This reaction is not only a key link in sugar metabolism, but also provides a biochemical basis for triglyceride detection. Its stable structure and clear catalytic mechanism make it an important biological enzyme for biochemical detection.

glycerol kinase

2, The core value of glycerol kinase in clinical testing

Serum triglyceride content is a key indicator for the diagnosis of hyperlipidemia, atherosclerosis, coronary heart disease and other diseases, and glycerol kinase is the core tool for accurate detection. The traditional chemical method for measuring triglycerides has problems such as cumbersome steps, low specificity, and susceptibility to interference, resulting in large detection errors and difficulty in meeting clinical needs. With the development of biotechnology, enzyme-linked immunosorbent assay kits have replaced chemical methods as the mainstream. Glycerol kinase, with its efficient catalytic activity, has become an indispensable key enzyme in the kit, directly determining detection sensitivity and accuracy, providing reliable data for doctors to judge patients' cardiovascular health.

3, Strict quality requirements for glycerol kinase in enzyme-linked immunosorbent assay kits

The enzyme-linked immunosorbent assay kit relies on the specificity and efficiency of enzymatic reactions, and requires high quality of glycerol kinase. Firstly, high purity is required. If there are impurities in the protein, it will interfere with the reaction and lead to distorted results; More importantly, strict control of interfering enzymes is crucial. NADH oxidase consumes NADH in the reaction system, affecting the detection signal. Catalase breaks down the hydrogen peroxide produced by the reaction, disrupting the stability of the system. If these two enzymes cannot be controlled at extremely low levels, it will seriously affect the detection accuracy and even lead to diagnostic misjudgment. Therefore, removing interfering enzymes is the core difficulty in the production of reagent kits.

4, Significant advantages of recombinant glycerol kinase compared to natural enzymes

To solve the problem of high interference enzyme content of glycerol kinase from natural bacterial strains, recombinant glycerol kinase produced by genetic engineering has outstanding advantages. When extracting and purifying natural enzymes, it is difficult to remove the symbiotic NADH oxidase and catalase, and even after multiple purifications, there are still residues that affect the detection results. Recombinant enzymes, through gene cloning, introduce the target gene into engineered bacteria for specific expression, which can efficiently synthesize the target protein while avoiding interference with enzyme production. Compared to natural enzymes, recombinant enzymes have higher purity and significantly reduce the content of interfering enzymes, fundamentally reducing detection interference, improving the accuracy and stability of reagent kits, providing higher quality enzyme preparations for clinical biochemical testing, and promoting the development of triglyceride detection technology.

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Hubei Xindesheng Material Technology Co., Ltd. has always adhered to the production and operation philosophy of "quality first, technology leadership" and "innovation and pursuit of excellence", continuously researching and developing reagents needed in the diagnostic field. Since its establishment in 2005, it has a history of nearly 20 years and is deeply trusted and supported by users. The diagnostic enzyme reagents independently developed and produced by Desheng have been sold to various parts of the world. If you have any related procurement needs in the near future, please feel free to contact us!