The working principle of the chromogenic substrate DAOS 83777-30-4 in triglyceride detection reagents

In the field of healthcare, blood lipid testing is crucial for assessing cardiovascular disease risk and developing reasonable health management plans. Among them, triglycerides, as an important component of blood lipids, have always been a focus of medical research for accurate detection. The chromogenic substrate DAOS reagent plays an indispensable role in triglyceride detection reagents, and its unique working principle provides strong guarantees for accurate detection.

1, Prerequisite for color development of DAOS molecular structure

DAOS has a unique molecular structure, which is the prerequisite for its ability to participate in color reactions and play a key role. Its molecules contain specific functional groups that endow DAOS with unique chemical properties. For example, hydroxyl and sulfopropyl groups in the molecule give DAOS a certain solubility and reactivity in solution. Meanwhile, the two groups on the benzene ring structure not only affect the electron cloud distribution of the molecule, but also enhance its stability. This stable and reactive molecular structure enables DAOS to accurately interact with other substances in subsequent chemical reactions, providing a solid framework for the smooth progress of color reactions.  

DAOS powder

2, DAOS color rendering triglycerides

In the process of triglyceride detection, the degree of color development of DAOS is directly related to the content of triglycerides in the sample, thus achieving quantitative detection. When the detection reagent is mixed with a sample containing triglycerides, a series of complex chemical reactions are initiated. Firstly, triglycerides in the sample undergo hydrolysis reactions under the action of specific enzymes, breaking down into glycerol and fatty acids. These decomposition products act as signaling molecules, further triggering subsequent chain reactions. During this process, DAOS specifically binds to other substances in the reaction system, causing a change in its molecular structure. This structural change causes a change in the absorption characteristics of DAOS towards light, resulting in a specific color. Moreover, there is a strict linear relationship between color depth and triglyceride content. That is to say, the higher the triglyceride content in the sample, the darker the color produced after the reaction; On the contrary, the lower the content, the lighter the color. By accurately measuring the color intensity of the solution after the reaction, the content of triglycerides in the sample can be accurately calculated, providing reliable diagnostic basis for doctors.

3, DAOS performance stability and precise detection

The stability of DAOS's own performance is a key factor in ensuring accurate and reliable detection results. In actual testing environments, factors such as temperature, pH value, and light exposure may have an impact on chemical reactions, thereby interfering with the testing results. However, DAOS has excellent chemical stability and can maintain its chemical properties unchanged over a wide temperature and pH range. This means that no matter how the detection environment changes, DAOS can stably participate in the color reaction, ensuring the repeatability and accuracy of the reaction. In addition, DAOS has high specificity as it only interacts with specific reactants, effectively avoiding the influence of other interfering substances and further improving the accuracy of detection.

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Hubei Xindesheng Material Technology Co., Ltd. has a complete solution for the research and production of chromogenic substrate such as DAOS, ensuring high product purity and good water solubility; At the same time, it can provide good after-sales service and customized service. If you have any recent purchasing needs, please click on the official website to learn more details or contact me!