Enzyme preparation preservation secret: Scientific protection of catalytic activity

In the era of booming biotechnology, enzyme preparations, as key biocatalysts, play an irreplaceable role in many fields such as precise medical detection and diagnosis, efficient synthesis of biopharmaceuticals, and improvement of food processing quality. However, the activity of enzymes is highly susceptible to interference from external environmental factors, and improper storage can lead to a decrease or even inactivation of their activity, seriously affecting their practical application effectiveness. Therefore, mastering scientific methods for preserving enzyme preparations is an important prerequisite for ensuring their optimal catalytic performance.

Freeze dried powder storage: precise control of multi ring nodes

Freeze dried powder is a common stable form of enzyme preparation, and its preservation involves multiple key steps such as preservation, transportation, and reconstitution. In terms of preservation, temperature and humidity are key factors. Storing freeze-dried powder at 4-8 ℃ can meet short-term storage needs. If long-term storage is required, it needs to be frozen in an environment of -20 ℃, while ensuring a dry storage environment to prevent moisture absorption, as moisture may cause conformational changes in enzymes, thereby affecting activity. During transportation, in order to maintain a low temperature environment, it is necessary to use ice packs with insulated boxes, and the temperature should be controlled at ≤ 10 ℃. The freeze-dried powder should also be packaged to prevent damage to the product caused by ice pack rupture. The reconstitution process cannot be ignored, and specialized enzyme buffer solutions such as SAHH enzyme buffer should be used. When reconstitution, it should be gently mixed to avoid the formation of bubbles, and direct sunlight should be avoided to prevent adverse effects of ultraviolet radiation on enzyme activity. If the enzyme solution after reconstitution is not used up, 20% glycerol can be added and stored at 4 ℃. Glycerol can reduce water activity and minimize enzyme denaturation and inactivation.

Liquid enzyme preservation: flexible response based on duration

The storage method of liquid enzymes should be flexibly selected according to the length of use time. For short-term use, i.e. use up within 1-3 months, the liquid enzyme can be stored in an environment of 4 ℃ -8 ℃ while maintaining a final concentration of 20% glycerol. Glycerol acts as a stabilizer here, helping to maintain the stability of enzyme molecular structure. When long-term storage is required, with a time span of 1-3 years, the method of packaging and freezing at -20 ℃ should be adopted. Packaging can avoid repeated freezing and thawing, as repeated freezing and thawing can cause enzyme molecules to undergo the formation and melting of ice crystals, resulting in the destruction of the enzyme's spatial structure and affecting its activity. In addition, there are some situations where the "red line" for liquid enzyme preservation must be strictly followed. Liquid enzymes should not be left directly at room temperature for more than 2 hours. Temperature fluctuations are significant at room temperature, and microorganisms are prone to grow, which may damage the enzyme's activity. At the same time, it is necessary to avoid mixing enzymes with heavy metals and strong acid-base compounds. Heavy metal ions may bind to certain functional groups in enzyme molecules, altering the structure of the enzyme; Strong acidity or alkalinity can break the chemical bonds in enzyme molecules, leading to enzyme denaturation and inactivation.

Whether it is freeze-dried powder or liquid enzyme, scientific preservation methods are the cornerstone to ensure their activity. In practical applications, researchers, production enterprises, and other parties should attach great importance to the preservation of enzyme preparations. Researchers follow the correct storage methods to ensure the accuracy and reliability of experimental data; Good preservation work by production enterprises can ensure product quality and enhance market competitiveness.

Hubei Xindesheng Material Technology Co., Ltd. has established a professional enzyme preparation research and development team to develop high-quality and stable enzyme preparation products. The professional technical team can answer any questions you may have during the use and storage of enzyme preparations at any time. If you have any recent purchasing needs, please click on the official website to learn more details or contact me directly!